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Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
 

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

Article DOI: 10.3791/63932-v • 08:59 min • May 5th, 2022
May 5th, 2022 •
1, 1
1Division of Newborn Medicine, Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School

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The present protocol describes preparing and utilizing mouse precision-cut lung slices to assess the airway and intrapulmonary arterial smooth muscle contractility in a nearly in vivo milieu.

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Precision-Cut Lung Slice Contractile Regulation Airway Smooth Muscle Intrapulmonary Arterial Smooth Muscle Ex Vivo Model Pulmonary Research Smooth Muscle Cell Activity In Vivo Phenotype Mechanistic Study Cellular Level Dissection Board Supine Position 25G Syringe Needles 70% Ethanol Chest Cavity Sternum Rib Cage Lung Lobes Collapse Trachea 1.2 Millimeters Diameter Hole Y-shaped IV Catheter Tip Adapter Port 3 Milliliter Syringe 0.5 Milliliters Of Air 1.5% Agar
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