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Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
 

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

Article DOI: 10.3791/50898-v • 11:22 min • February 24th, 2014
February 24th, 2014 •
1, 2, 3, 1, 1, 4, 1, 1
1Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology, Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

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Described here is a method to directly measure calcium sparks, the elementary units of Ca2+ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca2+ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca2+ signaling can be employed to assess muscle function in health and disease.

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Keywords: Calcium Sparks Skeletal Muscle Fibers Ryanodine Receptor Sarcoplasmic Reticulum Ca2+ Signaling Osmotic Stress Fluorescent Ca2+ Indicators Laser Scanning Confocal Microscopy Muscle Dystrophy Amyotrophic Lateral Sclerosis
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