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JoVE Journal
Immunology and Infection

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使用 ImageJ 自动分析 沙门氏菌 的细胞内表型
 
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使用 ImageJ 自动分析 沙门氏菌 的细胞内表型

Article DOI: 10.3791/64263-v 10:39 min August 9th, 2022
August 9th, 2022

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Riepilogo

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Click here for the English version.

沙门氏菌在沙门氏菌特异性液泡中和细胞质中游离(超复制)侵入并复制肠上皮细胞内。这里描述了一种基于高通量荧光显微镜的方案,通过 ImageJ 进行两次互补图像分析来量化沙门氏菌的细胞内表型,达到单细胞分辨率和评分。

Tags

免疫学和感染,第 186 期, 沙门氏菌,细胞内表型,空泡复制,胞质超复制,高通量方案,自动图像分析,荧光显微镜,ImageJ 脚本
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