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Biochemistry

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JoVE Journal Biochemistry
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
 

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

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The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.

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Keywords: Fluorogenic RNA Aptamers In Vitro Kinetics Cellular Kinetics Real-time Studies RNA Renaturation Thermocycler Plate Reader Injector Fluorescence Measurements Binding Buffer D-FHBI
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