Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Lumican Extraction from Amniotic Membrane and…
JoVE Journal
Medicine

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicine
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
 

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Article DOI: 10.3791/64460-v • 04:21 min • October 14th, 2022
October 14th, 2022 •
*1, *1, 1, 1,2
1Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, 2Biochemistry Department, Medicine Faculty, Universidad Nacional Autónoma de Mexico
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.

Tags

Keywords: Lumican Amniotic Membrane Protein Extraction Balanced Salt Solution Dispase II De-epithelialization Liquid Nitrogen Protease Inhibitor Centrifugation Storage Temperature
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter