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Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

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Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

DOI:

10.3791/64589-v

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06:32 min

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November 04, 2022

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Junli Chang*^1,2, Xingyuan Sun*^1,2, Xiaoping Ma², Peng Zhao², Binhao Shi², Yongjun Wang², Xianghui Han³, Yanping Yang²

¹Longhua Hospital,Shanghai University of Traditional Chinese Medicine, ²Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education, ³Institute of Chinese Traditional Surgery, Longhua Hospital,Shanghai University of Traditional Chinese Medicine

Capitoli

00:04Introduction
01:00Preparation of Prostate Cancer Cells for Injection
02:25Surgery for Intra‐Cardiac Injection of the Human Prostate Cancer Cells
05:03Results: Intra‐Cardiac Injection of Prostate Cancer Cells Induced Metastatic Lesions in Mice
06:04Conclusion

Summary

Traduzione automatica

Here, we present a protocol for the intra-cardiac injection of human prostate cancer cells to generate a mouse model with bone metastasis lesions.

Tags

Intra-cardiac Injection Prostate Cancer Cells Bone Metastasis Xenograft Mouse Model Luciferase-labeled PC3 Cells Cell Culture Trypsinization Cell Counting Centrifugation Cell Suspension Anesthesia Surgical Procedure Xiphoid Process Jugular Notch Intercostal Space Injection Point

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