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Immunology and Infection

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JoVE Journal Immunology and Infection
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
 

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

Article DOI: 10.3791/64634-v • 05:44 min • December 9th, 2022
December 9th, 2022 •
1,2
1The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences, Lund University, 2Department of Autoimmunity, BTB Emulsions

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To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.

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Keywords: Homogenization Method Antigen/adjuvant Emulsion Experimental Autoimmune Encephalomyelitis EAE CFA Slurry IFA Adjuvant Emulsion Preparation Autoimmune Disease Model Cancer Vaccines
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