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Neuroscience

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JoVE Journal Neuroscience
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
 

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Article DOI: 10.3791/64701-v • 08:25 min • April 21st, 2023
April 21st, 2023 •
1,2, 3, 1, 3, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology, University of Massachusetts Chan Medical School

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This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.

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Keywords: Intravital Imaging Fluorescent Protein Traumatic Brain Injury Cranial Window Two-photon Microscopy Neuronal Response Mechanical Stress Gene Of Interest Virus Promoter Cell Types Ophthalmic Ointment Midline Incision Periosteum Stereotaxic Frame TBI Impact Site Impactor Tip Dental Cement Lateral Muscles Cranial Window Implantation
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