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Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome
 

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line.

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Keywords: Cell-specific Ovarian Epigenome Ovarian Transcriptome Cell Isolation Cell Type-specific Cre Driver Nuclei Isolation Cell Sorting Paired Analysis Ovarian Aging Female Infertility
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