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Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
 

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Article DOI: 10.3791/64905-v • 11:58 min • January 6th, 2023
January 6th, 2023 •
1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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Organoid-fibroblast co-cultures provide a model to study the in vivo stem cell niche. Here, a protocol for esophageal organoid-fibroblast co-cultures is described. Additionally, whole mount imaging is used to visualize the fibroblast-organoid interaction.

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Functional Characterization Visualization Esophageal Fibroblasts Organoid Co-cultures Progenitor Cells Tissue Homeostasis Fibroblast Behavior Co-culture System Esophageal Progenitor Niche Organoid Clearing Cell Interactions Organoid Morphology Human Esophageal Organoids Cancer-associated Fibroblasts Dissociation Of Cells Viable Organoids Muscularis Externa Removal Esophagus Dissection Tissue Damage Prevention
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