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JoVE Journal Neuroscience
Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings
 

Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

Article DOI: 10.3791/64908-v • 11:05 min • April 21st, 2023
April 21st, 2023 •
1, 1, 1, 1, 1, 1
1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program, University of Southern California

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Presented here are methods providing detailed instructions for dissecting, dissociating, culturing, and patch-clamp recording from vestibular ganglion and spiral ganglion neurons of the inner ear.

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Keywords: Vestibular Spiral Ganglion Patch-clamp Neonatal Rodents Auditory Afferent Neurons Cell Bodies Ion Channels Neurotransmitter Receptors In Vitro Voltage-dependent Properties Cell Diversity Dissociation Otic Capsule Auditory Nerve Superior Ganglion Inferior Ganglion Peripheral Nerve Branch Utricle
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