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Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
 

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Article DOI: 10.3791/64911-v • 03:55 min • March 24th, 2023
March 24th, 2023 •
*1,2, *1,2, 3, 4,5, 2,6,7, 2,6
1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University
* These authors contributed equally

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This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

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Keywords: Modified Autologous Conditioned Serum (mACS) Autologous Serum Wound Healing Corneal Epithelium Ex Vivo Assessment Murine Model Blood-derived Product Tissue Regeneration Anti-inflammation Ocular Surface Disease Cytokine Randomized Controlled Trials
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