Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Culture of Piglet Intestinal 3D Organoids from…
JoVE Journal
Biology

Questo Contenuto è ad Accesso gratuito.

JoVE Journal Biology
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
 

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Article DOI: 10.3791/64917-v • 08:19 min • February 10th, 2023
February 10th, 2023 •
1,2, 1, 3, 2, 4, 1, 1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

Capitoli

Riepilogo
Automatic Translation

Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.

Tags

Keywords: Pig Intestinal Epithelium Intestinal Organoids 3D Organoids Cryopreserved Crypts Epithelial Crypts 2D Monolayers Nutrient Transport Barrier Function Host-microbe Interactions Jejunum Duodenum Ileum Colon Extracellular Matrix ECM Culture Medium Passaging Dissociation DMEM Rock Inhibitor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter