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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
 

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Article DOI: 10.3791/62438-v • 14:54 min • February 12th, 2022
February 12th, 2022 •
*1,2, *1,3, 1,2, 1,2
1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine, Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine, Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
* These authors contributed equally

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A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering.

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Keywords: Hepatocyte Organoids Liver Metabolism Detoxification Regeneration In Vitro Culture Genetic Modification Liver Perfusion Liver Digestion Single Cell Suspension Organoid Culture Metrogel Passage Genetic Manipulation
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