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量化肿瘤球状体模型中抗体依赖性细胞毒性:药物发现应用
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
DOI:

13:19 min

April 26, 2024

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Capitoli

  • 00:12Introduction
  • 01:07Setting up the JIMT-1 Enhanced Fluorescent Protein (EGFP) Spheroid Model
  • 03:43Coating the HCS Plate
  • 04:33Transfer of Spheroids to the HCS Plate
  • 04:46Pre-treatment of JIMT-1-EGFP Spheroids with Test Compounds
  • 05:14Induction of Antibody-Dependent Cellular Cytotoxicity (ADCC) by Adding the Effector Cells
  • 07:10Annexin V-647 Staining of Spheroids
  • 07:34Imaging
  • 11:43Representative Results
  • 12:52Conclusion

Summary

Traduzione automatica

在这里,我们提出了一种鉴定调节ADCC机制的化合物的方法,ADCC机制是抗肿瘤抗体的重要癌细胞杀伤机制。在曲妥珠单抗存在下,在乳腺癌细胞球状体中测量NK细胞的细胞毒性作用。图像分析可识别球状体中的活细胞和死细胞、杀伤细胞和靶细胞。

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