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Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
 
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Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

Article DOI: 10.3791/675-v • 17:42 min • February 13th, 2008
February 13th, 2008 •
1, 1
1Center for Neuroscience, University of California, Davis

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We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.

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Gene Gun Bullets Biolistic Transfection Neurons Slice Culture DNA Coated Particles Rat Hippocampal Slices Gene Gun Transfection Fluorescent Labeling Gold Particles Plastic Tubing Plastic Cartridges Bio-Rad Helios Gene Gun Tissue Slices
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