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A Method for Characterizing Embryogenesis in Arabidopsis
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Developmental Biology
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JoVE Journal Developmental Biology
A Method for Characterizing Embryogenesis in Arabidopsis

A Method for Characterizing Embryogenesis in Arabidopsis

DOI:
10.3791/55969-v

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10:24 min

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August 04, 2017

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1College of Life Sciences, Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement,Capital Normal University, 2College of Life Science,Shanxi Normal University

Chapters

  • 00:05Title
  • 00:44Preparation of Plant Material and Hoyer’s Solution
  • 03:40Ovule Separation and Clearance
  • 05:18Characterization of Embryo Patterning and Cell Identity via the Observation of Marker Protein Expression
  • 07:46Results: Embryogenesis Characterization in Arabidopsis
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.

Tags

EmbryogenesisArabidopsisPlant EmbryoPattern FormationModel OrganismSeed SterilizationSeed StratificationMS MediumHoyer’s SolutionOvule Pollination

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