Fumihiro Sugiyama Laboratory Animal Resource Center and Trans-Border Medical Research Center University of Tsukuba Biography Publications Institution JoVE Articles Fumihiro Sugiyama has not added a biography. If you are Fumihiro Sugiyama and would like to personalize this page please email our Author Liaison for assistance. Publications A Degron System Targeting Endogenous PD-1 Inhibits the Growth of Tumor Cells in Mice NAR Cancer. Jun, 2022 | Pubmed ID: 35734392 Birth of Mice from Meiotically Arrested Spermatocytes Following Biparental Meiosis in Halved Oocytes EMBO Reports. May, 2022 | Pubmed ID: 35587095 L17ER4: A Cell-permeable Attenuated Cationic Amphiphilic Lytic Peptide Bioorganic & Medicinal Chemistry. 05, 2022 | Pubmed ID: 35395514 Inducible Systemic Deletion in Mice Leads to Transient Body Weight Loss Upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen Storage International Journal of Molecular Sciences. Mar, 2022 | Pubmed ID: 35328622 In Vitro and in Vivo Functions of T Cells Produced in Complemented Thymi of Chimeric Mice Generated by Blastocyst Complementation Scientific Reports. 02, 2022 | Pubmed ID: 35217706 DAJIN Enables Multiplex Genotyping to Simultaneously Validate Intended and Unintended Target Genome Editing Outcomes PLoS Biology. 01, 2022 | Pubmed ID: 35041655 Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction Cancers. Aug, 2021 | Pubmed ID: 34439392 Relationships Between and Osteoclastogenesis Comparative Medicine. 08, 2021 | Pubmed ID: 34301346 Efficient Induction of Proximity-dependent Labelling by Biotin Feeding in BMAL1-BioID Knock-in Mice Journal of Biochemistry. Dec, 2021 | Pubmed ID: 33982090 EXOC1 Plays an Integral Role in Spermatogonia Pseudopod Elongation and Spermatocyte Stable Syncytium Formation in Mice ELife. 05, 2021 | Pubmed ID: 33973520 Disruption of Entire Locus Leads to Embryonic Lethality by Diminished Gene Expression and Enhanced P53 Pathway ELife. 05, 2021 | Pubmed ID: 33949947 Response to Correspondence on "Reproducibility of CRISPR-Cas9 Methods for Generation of Conditional Mouse Alleles: a Multi-center Evaluation" Genome Biology. 04, 2021 | Pubmed ID: 33827648 Induction of Mutant Allele in Neurons in Late Infancy Increases Sleep Need The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. 03, 2021 | Pubmed ID: 33558433 Majority of Alpha2,6-sialylated Glycans in the Adult Mouse Brain Exist in O-glycans: SALSA-MS Analysis for Knockout Mice of Alpha2,6-sialyltransferase Genes Glycobiology. 06, 2021 | Pubmed ID: 33242079 Characterization of a Bicistronic Knock-in Reporter Mouse Model for Investigating the Role of CABLES2 in Vivo Experimental Animals. Feb, 2021 | Pubmed ID: 32779618 Efficient Production of Large Deletion and Gene Fragment Knock-in Mice Mediated by Genome Editing with Cas9-mouse Cdt1 in Mouse Zygotes Methods (San Diego, Calif.). 07, 2021 | Pubmed ID: 32334080 Transcriptomic Evaluation of Pulmonary Fibrosis-Related Genes: Utilization of Transgenic Mice with Modifying P38 Signal in the Lungs International Journal of Molecular Sciences. Sep, 2020 | Pubmed ID: 32937976 Sleep Architecture in Mice Is Shaped by the Transcription Factor AP-2β Genetics. 11, 2020 | Pubmed ID: 32878901 Ablation of Ventral Midbrain/Pons GABA Neurons Induces Mania-like Behaviors with Altered Sleep Homeostasis and Dopamine DR-mediated Sleep Reduction IScience. Jun, 2020 | Pubmed ID: 32563157 Optimizing Charge Switching in Membrane Lytic Peptides for Endosomal Release of Biomacromolecules Angewandte Chemie (International Ed. in English). 11, 2020 | Pubmed ID: 32557993 Reverse Genetics Reveals Single Gene of Every Candidate on Hybrid Sterility, X Chromosome QTL 2 (Hstx2) Are Dispensable for Spermatogenesis Scientific Reports. 06, 2020 | Pubmed ID: 32493902 Ribosome Binding Protein GCN1 regulates the Cell Cycle and Cell Proliferation and is Essential for the Embryonic Development of Mice PLoS Genetics. 04, 2020 | Pubmed ID: 32324833 Generation of B6-Ddx4 , a Novel CreERT2 Knock-in Line, for Germ Cell Lineage by CRISPR/Cas9 Genesis (New York, N.Y. : 2000). 07, 2020 | Pubmed ID: 32293787 Highly Efficient CRISPR-targeting of the Murine Hipp11 Intergenic Region Supports Inducible Human Transgene Expression Molecular Biology Reports. Feb, 2020 | Pubmed ID: 31811500 Regulation of Bile Acid Metabolism in Mouse Models with Hydrophobic Bile Acid Composition Journal of Lipid Research. 01, 2020 | Pubmed ID: 31645370 Clec10a Regulates Mite-induced Dermatitis Science Immunology. 12, 2019 | Pubmed ID: 31811054 Non-invasive in Vivo Imaging of UCP1 Expression in Live Mice Via Near-infrared Fluorescent Protein IRFP720 PloS One. 2019 | Pubmed ID: 31730675 Reproducibility of CRISPR-Cas9 Methods for Generation of Conditional Mouse Alleles: a Multi-center Evaluation Genome Biology. 08, 2019 | Pubmed ID: 31446895 Generation of Bicistronic Reporter Knockin Mice for Visualizing Germ Layers Experimental Animals. Nov, 2019 | Pubmed ID: 31189761 Corrigendum to "Enhancing Endogenous Adenosine A Receptor Signaling Induces Slow-wave Sleep Without Affecting Body Temperature and Cardiovascular Function" [Neuropharmacology 144 (2019) 122-132] Neuropharmacology. Jul, 2019 | Pubmed ID: 31101360 The Chromatin-binding Protein Phf6 Restricts the Self-renewal of Hematopoietic Stem Cells Blood. 06, 2019 | Pubmed ID: 30917958 Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche Cell Stem Cell. 01, 2019 | Pubmed ID: 30581080 Enhancing Endogenous Adenosine A Receptor Signaling Induces Slow-wave Sleep Without Affecting Body Temperature and Cardiovascular Function Neuropharmacology. 01, 2019 | Pubmed ID: 30336152 Role of Anti-Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis Arthritis & Rheumatology (Hoboken, N.J.). 02, 2019 | Pubmed ID: 30079992 A Single Phosphorylation Site of SIK3 Regulates Daily Sleep Amounts and Sleep Need in Mice Proceedings of the National Academy of Sciences of the United States of America. 10, 2018 | Pubmed ID: 30254177 De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome American Journal of Human Genetics. 09, 2018 | Pubmed ID: 30146126 A Mutation in Transcription Factor MAFB Causes Focal Segmental Glomerulosclerosis with Duane Retraction Syndrome Kidney International. 08, 2018 | Pubmed ID: 29779709 Enhanced Antibody Responses in a Novel NOG Transgenic Mouse with Restored Lymph Node Organogenesis Frontiers in Immunology. 2017 | Pubmed ID: 29387068 Simple Generation of Hairless Mice for in Vivo Imaging Experimental Animals. Oct, 2017 | Pubmed ID: 28717054 Nrf2 Improves Leptin and Insulin Resistance Provoked by Hypothalamic Oxidative Stress Cell Reports. 02, 2017 | Pubmed ID: 28228267 SHISA6 Confers Resistance to Differentiation-Promoting Wnt/β-Catenin Signaling in Mouse Spermatogenic Stem Cells Stem Cell Reports. 03, 2017 | Pubmed ID: 28196692 Angiodysplasia in Embryo Lacking Protein Arginine Methyltransferase 1 in Vascular Endothelial Cells Journal of Biochemistry. Mar, 2017 | Pubmed ID: 28003433 Forward-genetics Analysis of Sleep in Randomly Mutagenized Mice Nature. 11, 2016 | Pubmed ID: 27806374 H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells Stem Cell Reports. 06, 2016 | Pubmed ID: 27237376 Germline Recombination in a Novel Cre Transgenic Line, Prl3b1-Cre Mouse Genesis (New York, N.Y. : 2000). 07, 2016 | Pubmed ID: 27124574 Generation of CRISPR/Cas9-mediated Bicistronic Knock-in Ins1-cre Driver Mice Experimental Animals. Jul, 2016 | Pubmed ID: 27053096 Sialylation Converts Arthritogenic IgG into Inhibitors of Collagen-induced Arthritis Nature Communications. Apr, 2016 | Pubmed ID: 27046227 β-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development PloS One. 2016 | Pubmed ID: 26901059 Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells Stem Cells (Dayton, Ohio). Feb, 2016 | Pubmed ID: 26523946 Peri-implantation Lethality in Mice Carrying Megabase-scale Deletion on 5qc3.3 is Caused by Exoc1 Null Mutation Scientific Reports. Sep, 2015 | Pubmed ID: 26346620 Spatial Interplay Between Polycomb and Trithorax Complexes Controls Transcriptional Activity in T Lymphocytes Molecular and Cellular Biology. Nov, 2015 | Pubmed ID: 26324324 Erythropoiesis and Blood Pressure Are Regulated Via AT1 Receptor by Distinctive Pathways PloS One. 2015 | Pubmed ID: 26107632 Simple Generation of Albino C57BL/6J Mice with G291T Mutation in the Tyrosinase Gene by the CRISPR/Cas9 System Mammalian Genome : Official Journal of the International Mammalian Genome Society. Aug, 2014 | Pubmed ID: 24879364 Generation and Characterization of Ins1-cre-driver C57BL/6N for Exclusive Pancreatic Beta Cell-specific Cre-loxP Recombination Experimental Animals. 2014 | Pubmed ID: 24770644 A Mouse Renin Distal Enhancer is Essential for Blood Pressure Homeostasis in BAC-rescued Renin-null Mutant Mice Journal of Receptor and Signal Transduction Research. Oct, 2014 | Pubmed ID: 24734888 Truncated Cables1 Causes Agenesis of the Corpus Callosum in Mice Laboratory Investigation; a Journal of Technical Methods and Pathology. Mar, 2014 | Pubmed ID: 24336072 Novel ROSA26 Cre-reporter Knock-in C57BL/6N Mice Exhibiting Green Emission Before and Red Emission After Cre-mediated Recombination Experimental Animals. 2013 | Pubmed ID: 24172193 Effect of Lactation on Postpartum Cardiac Function of Pregnancy-associated Hypertensive Mice Endocrinology. Feb, 2013 | Pubmed ID: 23254193 Short-term Suppression of the Renin-angiotensin System in Mice Associated with Hypertension During Pregnancy Molecular Medicine Reports. 07, 2012 | Pubmed ID: 22552605 Pregnancy-associated Homeostasis and Dysregulation: Lessons from Genetically Modified Animal Models Journal of Biochemistry. Jul, 2011 | Pubmed ID: 21613291 Retrotransposon-mediated Fgf5(go-Utr) Mutant Mice with Long Pelage Hair Experimental Animals. 2011 | Pubmed ID: 21512271 Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-based Multiplex Fluorescence Immunoassay Clinical and Vaccine Immunology : CVI. May, 2011 | Pubmed ID: 21430123 Genome-wide Analysis of Target Genes Regulated by HoxB4 in Hematopoietic Stem and Progenitor Cells Developing from Embryonic Stem Cells Blood. Apr, 2011 | Pubmed ID: 21343615 Effect of Different Culture Conditions on Establishment of Embryonic Stem Cells from BALB/cAJ and NZB/BINJ Mice Cellular Reprogramming. Dec, 2010 | Pubmed ID: 20977302 A Novel Locus on Proximal Chromosome 18 Associated with Agenesis of the Corpus Callosum in Mice Mammalian Genome : Official Journal of the International Mammalian Genome Society. Dec, 2010 | Pubmed ID: 20967545 Autotaxin/lysophospholipase D-mediated Lysophosphatidic Acid Signaling is Required to Form Distinctive Large Lysosomes in the Visceral Endoderm Cells of the Mouse Yolk Sac The Journal of Biological Chemistry. Nov, 2009 | Pubmed ID: 19808661 Genetic Analysis of Blood Pressure in 8 Mouse Intercross Populations Hypertension (Dallas, Tex. : 1979). Oct, 2009 | Pubmed ID: 19652078 Angiotensin Type 1 Receptor Blockade Prevents Cardiac Remodeling in Mice with Pregnancy-associated Hypertension Hypertension Research : Official Journal of the Japanese Society of Hypertension. Dec, 2008 | Pubmed ID: 19139606 Embryonic Stem Cells Derived from C57BL/6J and C57BL/6N Mice Comparative Medicine. Aug, 2008 | Pubmed ID: 18724776 Deterioration of Atherosclerosis in Mice Lacking Angiotensin II Type 1A Receptor in Bone Marrow-derived Cells Laboratory Investigation; a Journal of Technical Methods and Pathology. Jul, 2008 | Pubmed ID: 18490898 Novel Embryonic Stem Cells Expressing TdKaede Protein Photoconvertible from Green to Red Fluorescence International Journal of Molecular Medicine. Oct, 2007 | Pubmed ID: 17786273 Quantitative Trait Loci Associated with Blood Pressure of Metabolic Syndrome in the Progeny of NZO/HILtJxC3H/HeJ Intercrosses Mammalian Genome : Official Journal of the International Mammalian Genome Society. Aug, 2007 | Pubmed ID: 17641813 Development of ELISA Using Recombinant Antigens for Specific Detection of Mouse Parvovirus Infection Experimental Animals. Apr, 2006 | Pubmed ID: 16651694 Uterine Sensitization-associated Gene-1 (USAG-1), a Novel BMP Antagonist Expressed in the Kidney, Accelerates Tubular Injury The Journal of Clinical Investigation. Jan, 2006 | Pubmed ID: 16341262 A Novel Subpopulation Lacking Oct4 Expression in the Testicular Side Population International Journal of Molecular Medicine. Jan, 2006 | Pubmed ID: 16328007 Enhanced Erythropoiesis Mediated by Activation of the Renin-angiotensin System Via Angiotensin II Type 1a Receptor FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Dec, 2005 | Pubmed ID: 16223784 Redox Imbalance in Cystine/glutamate Transporter-deficient Mice The Journal of Biological Chemistry. Nov, 2005 | Pubmed ID: 16144837 Parvovirus Nonstructural Proteins Induce an Epigenetic Modification Through Histone Acetylation in Host Genes and Revert Tumor Malignancy to Benignancy Journal of Virology. Jul, 2005 | Pubmed ID: 15994782 Establishment of a New Embryonic Stem Cell Line Derived from C57BL/6 Mouse Expressing EGFP Ubiquitously Genesis (New York, N.Y. : 2000). May, 2005 | Pubmed ID: 15830376 Neurochondrin Negatively Regulates CaMKII Phosphorylation, and Nervous System-specific Gene Disruption Results in Epileptic Seizure The Journal of Biological Chemistry. May, 2005 | Pubmed ID: 15790563 Differential Roles of Renin and Angiotensinogen in the Feto-maternal Interface in the Development of Complications of Pregnancy Molecular Endocrinology (Baltimore, Md.). May, 2005 | Pubmed ID: 15695374 Blood Pressure in 15 Inbred Mouse Strains and Its Lack of Relation with Obesity and Insulin Resistance in the Progeny of an NZO/HILtJ X C3H/HeJ Intercross Mammalian Genome : Official Journal of the International Mammalian Genome Society. Dec, 2004 | Pubmed ID: 15599552 Regulatory Roles for APJ, a Seven-transmembrane Receptor Related to Angiotensin-type 1 Receptor in Blood Pressure in Vivo The Journal of Biological Chemistry. Jun, 2004 | Pubmed ID: 15087458 An Essential Role for Angiotensin II Type 1a Receptor in Pregnancy-associated Hypertension with Intrauterine Growth Retardation FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Feb, 2004 | Pubmed ID: 14688210 The Endothelin Receptor Antagonist Ameliorates the Hypertensive Phenotypes of Transgenic Hypertensive Mice with Renin-angiotensin Genes and Discloses Roles of Organ Specific Activation of Endothelin System in Transgenic Mice Life Sciences. Jan, 2004 | Pubmed ID: 14687651 Hypothalamic Orexin Neurons Regulate Arousal According to Energy Balance in Mice Neuron. Jun, 2003 | Pubmed ID: 12797956 Elimination of Pasteurella Pneumotropica from a Contaminated Mouse Colony by Oral Administration of Enrofloxacin Experimental Animals. Jul, 2002 | Pubmed ID: 12221935 Intraocular Pressure in Genetically Distinct Mice: an Update and Strain Survey BMC Genetics. 2001 | Pubmed ID: 11532192 Propagation of Rat Parvovirus in Thymic Lymphoma Cell Line C58(NT)d and Subsequent Appearance of a Resistant Cell Clone After Lytic Infection Journal of Virology. Apr, 2001 | Pubmed ID: 11264385 Targeted Expression of Baculovirus P35 Caspase Inhibitor in Oligodendrocytes Protects Mice Against Autoimmune-mediated Demyelination The EMBO Journal. Feb, 2000 | Pubmed ID: 10654933 Zygote micro-injectie voor het maken van gencassette knock-in en flox-allelen bij muizen Yoko Tanimoto*1, Natsuki Mikami*1,2, Miyuki Ishida1, Natsumi Iki1, Kanako Kato1, Fumihiro Sugiyama1, Satoru Takahashi1, Seiya Mizuno1 1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba JoVE 64161 발생학
Zygote micro-injectie voor het maken van gencassette knock-in en flox-allelen bij muizen Yoko Tanimoto*1, Natsuki Mikami*1,2, Miyuki Ishida1, Natsumi Iki1, Kanako Kato1, Fumihiro Sugiyama1, Satoru Takahashi1, Seiya Mizuno1 1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba JoVE 64161 발생학