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Journal / Neuroscience / A Thin-skull Window Technique for Chronic…
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Neuroscience

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JoVE Journal Neuroscience
A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation
 

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

Article DOI: 10.3791/2059-v • 12:08 min • September 19th, 2010
September 19th, 2010 •
*1, *2, 1, 2, 1
1Center for Neural Development and Disease, Department of Neurology, Child Neurology Division, University of Rochester, 2Department of Neurobiology and Anatomy, University of Rochester
* These authors contributed equally

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요약
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We describe a method for repeatedly visualizing murine microglia and circulating monocytes in vivo over hours, days or weeks using transcranial two-photon microscopy. We demonstrate how to prepare a thinned-skull window that allows intermittent observation of quiescent microglia that can be activated by adjacent stereotactic injection of the HIV-1 regulatory protein Tat.

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Thin-skull Window Technique Chronic Two-photon In Vivo Imaging Murine Microglia Neuroinflammation Open-skull Technique Thinned-skull Approach Microglial Activation Chronic Imaging Tissue Injury Imaging Sessions Two-photon Microscopy Stable Cortical Window Glass Coverslip Translucent Immunologically Inert CX3CR1 GFP/+ Mice Pial Surface Stereotactic Brain Injury
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