Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation

Journal / Neuroscience /

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Article DOI: 10.3791/53582-v • 10:04 min • January 12th, 2016

January 12th, 2016 •

This paper describes a method by which the vascular architecture in the brain can be quantified using in vivo and ex vivo two-photon microscopy.

Quantification Cerebral Vascular Architecture Two-photon Microscopy Mouse Model HIV-induced Neuroinflammation Cortical Capillary Networks Tat Virotoxin HIV Associated Neurocognitive Disorders Capillary Morphology Physiological Accuracy Multiple Capillary Morphological Parameters Vascular Changes Alzheimer's Disease Artificial Tear Gel Anesthetized Mouse Electric Razor Povidone Iodine Solution Sterilize The Scalp Light Microscope Parietal Bones Caudal Frontal Bones Bregma Point Ferric Chloride Solution Dried Membrane Forceps Head Plate Window