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Hsp104纯化，蛋白质Disaggregase
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JoVE 신문 생물학
Purification of Hsp104, a Protein Disaggregase
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Hsp104纯化，蛋白质Disaggregase

DOI:
10.3791/3190-v

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07:17 min

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September 30, 2011

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, ,
1Department of Biochemistry and Biophysics,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:07Cell Harvest and Lysis
  • 01:55Ni-affinity Purification of Hsp104
  • 03:16FPLC Purification of Hsp104
  • 05:14Representative Results for Hsp104 Purification and Assessment of Disaggregation Activity
  • 07:04Conclusion

Summary

자동 번역

자동 번역

在这里，我们描述一个高度活跃Hsp104，一个六聚AAA +从酵母中的蛋白质，其中夫妇ATP水解蛋白分解净化的协议。该计划利用从亲和纯化His6标签的​​构造<em> E。大肠杆菌</em>其次，His6标签去除TEV蛋白酶，体积排阻色谱阴离子交换层析。

Tags

PurificationHsp104Protein DisaggregaseHexameric AAA+ ProteinATP HydrolysisProtein DisaggregationRenaturationDisordered AggregatesHeat ShockCross-beta Amyloid FibrilsPrionsPhenotypic VariationPreamyloid OligomersE. Coli Orthologue ClpBHsp104 OrthologuesAnimal CellsTherapeutic AgentNeurodegenerative DiseasesMisfolding Of ProteinsToxic Preamyloid OligomersAmyloid Fibrils

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