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Journal / Biology / Genome Editing with CompoZr Custom Zinc Finger…
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Biology

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Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
 

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Article DOI: 10.3791/3304-v • 09:11 min • June 14th, 2012
June 14th, 2012 •
1, 1, 1, 1, 1, 1
1Emerging Technologies, Sigma Life Science

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요약
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The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.

Tags

Genome Editing CompoZr Custom Zinc Finger Nucleases Gene Function Genetic Basis Of Disease Traditional Gene Editing Methods Chemical-based Mutagenesis Random Integration Of DNA Sequences Synthetic Sequences Aberrant Culture Conditions Transient ZFN Expression Precise Gene Editing Highly Efficient Manner Zinc Finger Nucleases Double-stranded DNA (dsDNA) CompoZr ZFN Unit DNA-cleavage Domains Double-strand Break (DSB) Non-homologous End-joining (NHEJ) DNA Repair Pathway Small Insertions And Deletions (indels) Frameshift Functional Knockout
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