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Chapters
- 00:05Title
- 01:15Culturing Mouse Embryonic Stem Cells (Timing: 3 days)
- 02:34Neural Induction (Timing: 2 days)
- 04:48Motor Neuron Specification (Timing: 5 days)
- 05:24Preparation of Poly-DL-Ornithine/Laminin Coated Coverslips (Timing: 2 days)
- 06:18Axon Elongation (Timing: 2 days)
- 07:47Results: mES Cell Differentiation into Motor Neurons
- 09:12Conclusion
Nous avons développé un nouveau protocole pour améliorer l'efficacité de la différenciation in vitro de cellules souches embryonnaires de souris en neurones moteurs. Les cellules ES différenciées acquis motoneurones dispose comme en témoigne l'expression de marqueurs neuronaux et des neurones moteurs en utilisant des techniques d'immunohistochimie.
Tags
Mouse Embryonic Stem CellsMotor NeuronsDifferentiationRetinoic AcidSonic HedgehogDisease MechanismsDrug CompoundsSpinal Muscular AtrophyAmyotrophic Lateral SclerosisProtocolsNeuralization ProcessNogginFibroblast Growth FactorsFGF SignalingNeurogenesisAnterior Neural PatterningPosterior Neural Identity
