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Chapters
- 00:05Title
- 01:15Culturing Mouse Embryonic Stem Cells (Timing: 3 days)
- 02:34Neural Induction (Timing: 2 days)
- 04:48Motor Neuron Specification (Timing: 5 days)
- 05:24Preparation of Poly-DL-Ornithine/Laminin Coated Coverslips (Timing: 2 days)
- 06:18Axon Elongation (Timing: 2 days)
- 07:47Results: mES Cell Differentiation into Motor Neurons
- 09:12Conclusion
Hemos desarrollado un nuevo protocolo para mejorar la eficiencia de la diferenciación in vitro de células madre embrionarias de ratón en neuronas motoras. Las células madre embrionarias diferenciadas adquirida cuenta con las neuronas motoras como lo demuestra la expresión de marcadores neuronales de neuronas y el motor mediante técnicas de inmunohistoquímica.
Tags
Mouse Embryonic Stem CellsMotor NeuronsDifferentiationRetinoic AcidSonic HedgehogDisease MechanismsDrug CompoundsSpinal Muscular AtrophyAmyotrophic Lateral SclerosisProtocolsNeuralization ProcessNogginFibroblast Growth FactorsFGF SignalingNeurogenesisAnterior Neural PatterningPosterior Neural Identity
