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饲养和注射<em>烟草天蛾</em>幼虫评估细菌的毒力
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
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饲养和注射<em>烟草天蛾</em>幼虫评估细菌的毒力

DOI:
10.3791/4295-v

•

09:04 min

•

December 11, 2012

•

,
1Department of Bacteriology,University of Wisconsin-Madison

Chapters

  • 00:05Title
  • 01:06Insect Egg Sterilization and Rearing
  • 04:06Preparation of Bacteria for Injection
  • 04:44Injection of 4th Instar Larvae
  • 07:46Results: Representative Insect Mortality Assay
  • 08:40Conclusion

Summary

자동 번역

자동 번역

这里所描述的方法采用直接注射昆虫病原细菌进入血淋巴<em>烟草天蛾</em>昆虫的幼虫。<em> M.天蛾</em是市售和充分研究昆虫。因此，此方法是一种简单的方法来从一个或两个合作伙伴的角度分析宿主细菌的相互作用。

Tags

Manduca SextaTobacco HornwormAgricultural PestSolanaceous PlantsEntomopathogenic BacteriaInsect Immune SystemGenome SequenceModel OrganismHost-microbe InteractionsPathogenesisLarvae InjectionVirulence CharacteristicsBacterial SpeciesMutantsTime To Insect DeathXenorhabdus SpeciesPhotorhabdus SpeciesNematode VectorsInsect Hemolymph

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