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RNA secundaire structuur Voorspelling behulp van High-throughput SHAPE
 
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RNA secundaire structuur Voorspelling behulp van High-throughput SHAPE

Article DOI: 10.3791/50243-v 13:42 min May 31st, 2013
May 31st, 2013

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High-throughput selectieve 2-hydroxyl acylering geanalyseerd door primerverlenging (SHAPE) gebruikt een nieuwe chemische indringende techniek, reverse transcriptie, capillaire elektroforese en secundaire structuur predictie software om de structuren van RNA bepalen van enkele honderden tot enkele duizenden nucleotiden aan nucleotide resolutie.

Tags

Genetics Molecular Biology Biochemistry Virology Cancer Biology Medicine Genomics Nucleic Acid Probes Probes RNA RNA High-throughput SHAPE capillaire elektroforese structuur van RNA RNA sonderen RNA vouwen secundaire structuur DNA nucleïnezuren electropherogram synthese transcriptie hoge doorvoer sequentiebepaling
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