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Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
 

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Article DOI: 10.3791/50448-v • 11:40 min • June 25th, 2013
June 25th, 2013 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University

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요약
Automatic Translation

The yeast mitochondrial nucleoid protein, Mgm101, is a Rad52-type recombination protein that forms large oligomeric rings. A protocol is described to prepare soluble recombinant Mgm101 using the Maltose Binding Protein (MBP)-tagging strategy coupled with cation exchange and size exclusion chromatography.

Tags

Mgm101 Recombination Protein MBP-based Tagging Strategy Maintenance Of Mitochondrial DNA Recombinational Repair Rad52-type Recombination Protein Family Annealing Of Homologous Single Stranded DNA Characterization Of Mgm101 Preparation Of Recombinant Mgm101 Maltose Binding Protein (MBP) Escherichia Coli Expression System Amylose Affinity Chromatography Proteolytic Cleavage Cationic Exchange Chromatography Size Exclusion Chromatography Yield Of Mgm101 Minimal Contamination Of DNA Biochemical Analysis Structural Analysis Single Particle Image Analysis
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