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Bioengineering

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JoVE Journal Bioengineering
Transplantation af induceret pluripotente stamcelle-afledt Mesoangioblast-lignende Myogenic Stamfadere i Mouse Modeller af muskel regenerering
 
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Transplantation af induceret pluripotente stamcelle-afledt Mesoangioblast-lignende Myogenic Stamfadere i Mouse Modeller af muskel regenerering

Article DOI: 10.3791/50532-v • 10:03 min • January 20th, 2014
January 20th, 2014 •
*1, *1, 1, 1,2
1Department of Cell and Developmental Biology, University College London, 2Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Hospital
* These authors contributed equally

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요약
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Induceret pluripotente stamceller (iPSC) afledte myogen forfædre er lovende kandidater til celleterapi strategier til behandling af muskeldystrofier. Denne protokol beskriver transplantation og funktionelle målinger, der kræves for at evaluere engraftment og differentiering af iPSC-afledte mesoangioblasts (en type muskel stamfadere) i musemodeller af akut og kronisk muskel regenerering.

Tags

Bioengineering Problem 83 Skeletmuskulatur Muskelceller Muskelfibre Skelet Pericytes Stamceller Induceret Pluripotente stamceller (iPSCs) Muskeldystrofier Celle differentiering dyremodeller muskel stamceller / stamceller mesoangioblasts muskel regenerering iPSC-afledte mesoangioblasts (IDEMs)
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