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Immunology and Infection

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JoVE Journal Immunology and Infection
Het identificeren van DNA-mutaties in Gezuiverd hematopoietische stamcellen / progenitorcellen
 
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Het identificeren van DNA-mutaties in Gezuiverd hematopoietische stamcellen / progenitorcellen

Article DOI: 10.3791/50752-v • 11:06 min • February 24th, 2014
February 24th, 2014 •
1, 1,2, 1, 3, 4,5, 2, 2,5, 1,2,5
1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio

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Hier beschrijven we een in vivo mutagenese test voor kleine aantallen hematopoietische cellen gezuiverd met de Lacl transgene muismodel. De Lacl gen kan worden geïsoleerd om de frequentie, locatie en soort DNA mutanten spontaan ontstaan ​​of na blootstelling aan genotoxinen bepalen.

Tags

Infectie , hematopoëtische stam / progenitorcellen, DNA-mutaties,
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