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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifisere DNA Mutasjoner i Purified Hematopoietic Stem / stamceller
 
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Identifisere DNA Mutasjoner i Purified Hematopoietic Stem / stamceller

Article DOI: 10.3791/50752-v • 11:06 min • February 24th, 2014
February 24th, 2014 •
1, 1,2, 1, 3, 4,5, 2, 2,5, 1,2,5
1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio

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Her beskriver vi en in vivo-mutagenese assay for små antall av hematopoetiske celler renset ved hjelp av laci transgen musemodell. Den laci-genet kan bli isolert for å bestemme frekvensen, plasseringen og typen av DNA-mutanter spontant oppstått eller etter eksponering for genotoxins.

Tags

Infeksjon , blodkreft stamceller / stamceller, DNA mutasjoner,
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