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JoVE Journal Biology
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
 

Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells

Article DOI: 10.3791/50819-v • 07:34 min • January 10th, 2014
January 10th, 2014 •
1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine

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The material here describes a method developed to preserve the three-dimensional chromatin structure of testicular germ cells. This has been termed the three-dimensional (3D) slide method. This method improves sensitivity for detection of subnuclear structures and is applicable for immunofluorescence, DNA, and RNA fluorescence in situ hybridization (FISH).

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Keywords: Three-dimensional Chromatin Architecture Testicular Germ Cells Slide Preparation Method Permeabilization Fixation Cytospin Immunofluorescence DNA FISH RNA FISH Cot-1 RNA FISH Germ Cell Differentiation
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