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Beoordeling van Calcium Sparks in Intact Skeletal spiervezels
 
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Beoordeling van Calcium Sparks in Intact Skeletal spiervezels

Article DOI: 10.3791/50898-v • 11:22 min • February 24th, 2014
February 24th, 2014 •
1, 2, 3, 1, 1, 4, 1, 1
1Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology, Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

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Hier beschreven is een methode om direct te meten calcium vonken, de elementaire eenheden van Ca2 + vrij van sarcoplasmatisch reticulum in intacte spiervezels. Deze methode maakt gebruik van osmotische stress-gemedieerde activering van Ca 2 + vrijlating uit ryanodinereceptor in geïsoleerde spiervezels. De dynamiek en homeostatische aantal intracellulaire Ca2 +-signalen kunnen worden gebruikt om spierfunctie in gezondheid en ziekte te beoordelen.

Tags

Fysiologie flexor digitorm brevis (FDB) sarcoplasmatisch reticulum SR Ca calcium signalering ryanodinereceptor confocale beeldvorming spierfysiologie
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