JoVE Journal
Environment
Environment
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
More than half of proteins are small proteins (molecular mass <200 kDa) that are challenging for both electron microscope imaging and three-dimensional reconstructions. Optimized negative staining is a robust and high-throughput protocol to obtain high contrast and relatively high resolution (~1 nm) images of small asymmetric proteins or complexes under different physiological conditions.