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1Genetics and Genomic Sciences Graduate Program,University of Alabama at Birmingham, 2Birmingham Veterans Affairs Medical Center, 3Department of Pathology,University of Alabama at Birmingham, 4Department of Biomedical Engineering,University of Alabama at Birmingham, 5Department of Medicine,University of Alabama at Birmingham
Chapters
- 00:05Title
- 01:50HUVEC Layer Preparation
- 03:07Purified Ligand Coating
- 05:11Neutrophil Separation
- 08:33Flow Chamber Adhesion Assay
- 10:20Results: The Flow Chamber Assay Enables Analyses of Adhesive Properties of Human Peripheral Blood Neutrophils to HUVECs and Purified Ligand Substrates
- 11:49Conclusion
È riportato un metodo di quantificazione neutrofili. Questo metodo crea un ambiente di flusso dinamico simile a quello riscontrato in un vaso sanguigno. Permette la ricerca di neutrofili adesione a entrambi le molecole purificate di adesione (ligando) o substrato di cellule endoteliali (HUVEC) in un contesto simile all'ambiente in vivo con lo stress puro.
