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Oprensning af cystisk fibrose Ledningsevne regulator protein udtrykt i<em> Saccharomyces cerevisiae</em
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Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

Oprensning af cystisk fibrose Ledningsevne regulator protein udtrykt i<em> Saccharomyces cerevisiae</em

DOI:
10.3791/51447-v

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15:12 min

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May 10, 2014

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, , ,
1Faculty of Life Sciences,University of Manchester

Chapters

  • 00:05Title
  • 01:46Solubilization of Microsomes
  • 03:49Nickel-affinity Purification of CFTR
  • 05:33Gel Permeation Chromatography (GPC) Purification of CFTR
  • 06:57Reconstitution of CFTR
  • 07:51Measurement of ATPase Activity
  • 09:35Results: Isolation of Milligram Quantities of CFTR by Two Methods
  • 14:28Conclusion

Summary

자동 번역

자동 번역

Heterolog ekspression og oprensning af cystisk fibrose transmembran konduktans regulator (CFTR) er betydelige udfordringer og begrænsende faktorer i udviklingen af ​​lægemiddelbehandlinger for cystisk fibrose. Denne protokol beskriver to metoder til isolering af milligram mængder af CFTR egnet til funktionelle og strukturelle studier.

Tags

Keywords: Cystic FibrosisCFTR ProteinMembrane Protein PurificationSaccharomyces CerevisiaeDetergent SolubilizationDDMLPG-14ATPase ActivityPost-translational ModificationsStructural Analysis

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