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Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
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1Department of Infectomics and Molecular Pathogenesis,Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Molecular Biomedicine,Center for Research and Advanced Studies of the National Polytechnic Institute, 3Agency for Knowledge Commercialization,Center for Research and Advanced Studies of the National Polytechnic Institute
Chapters
- 00:05Title
- 02:43Preparation of Cultures and Trophozoite Lysates
- 04:00Preparation of Trophozoite Secreted Products
- 04:58Interaction of MDCK Cells with Trophozoites, Trophozoite Lysates or Secreted Products
- 07:33Measurement of Transepithelial Electrical Resistance (TER)
- 08:42Results: Epithelial Damage by Entamoeba histolytica
- 10:27Conclusion
Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.
