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Biochemischen Assays zur Analyse von Aktivitäten von ATP-abhängigen Chromatin-Remodeling-Enzyme
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Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
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Biochemischen Assays zur Analyse von Aktivitäten von ATP-abhängigen Chromatin-Remodeling-Enzyme

DOI:
10.3791/51721-v

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10:14 min

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October 25, 2014

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, , ,
1Stowers Institute for Medical Research, 2Department of Biochemistry & Molecular Biology,Kansas University Medical Center

Chapters

  • 00:05Title
  • 01:15ATP-dependent Nucleosome Remodeling Assays
  • 02:55ATP-dependent Nucleosome Sliding Assays
  • 05:11DNA- and Nucleosome-dependent ATPase Assays
  • 07:15Defining Biochemical Activities of the INO80 Complex and INO80 Subcomplexes
  • 09:17Conclusion

Summary

자동 번역

자동 번역

Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.

Tags

Keywords: ATP-dependent Chromatin RemodelingSNF2 Family ATPasesNucleosome SlidingNucleosome BindingDNA-dependent ATPase ActivityNucleosome-dependent ATPase ActivityElectrophoretic Mobility Shift Assay (EMSA)Thin Layer Chromatography (TLC)INO80 Chromatin Remodeling Complex

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