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JoVE Journal Chemistry
Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
 

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Article DOI: 10.3791/51840-v • 13:06 min • November 20th, 2014
November 20th, 2014 •
1,2, 1, 1,3, 1,2, 1
1Complex Carbohydrate Research Center, University of Georgia, 2Department of Biochemistry and Molecular Biology, University of Georgia, 3Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

챕터

요약
Automatic Translation

In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.

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Keywords: In-gel Reductive β-elimination O-linked Glycans Sulfo-glycans SDS-PAGE Mass Spectrometry Glycomic Analysis Sulfoglycomic Analysis Glycoproteins Permethylation Phase-partition
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