Journal
/
생물학
/
核酸，蛋白质与甲基转移和辅酶类似物序列特异性标签
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Please note that all translations are automatically generated. Click here for the English version.

核酸，蛋白质与甲基转移和辅酶类似物序列特异性标签

DOI:
10.3791/52014-v

•

12:07 min

•

November 22, 2014

•

, , , ,
1Institute of Organic Chemistry, Department of Chemistry,RWTH Aachen University

Chapters

  • 00:05Title
  • 02:26One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
  • 05:07Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
  • 08:41Results: Gel Analyses for SMILing and mTAG Reactions
  • 11:04Conclusion

Summary

자동 번역

자동 번역

DNA和蛋白质序列特异性标记的亲和力，或使用DNA或蛋白质的甲基和合成的辅助因子的类似物的荧光报告基团。取决于酶，氮丙啶或双活化的辅助因子类似物的辅因子特异性的用于一个或两个步骤的标签。

Tags

MethyltransferasesS-Adenosyl-l-methionine (AdoMet Or SAM)DNA MethylationRNA MethylationProtein MethylationAziridine CofactorsSMILing DNAMTAGDouble-activated AdoMet AnaloguesHistone MethylationClick ChemistryDNA GenotypingMethylation Detection

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

小学组织两个胸苷类似物的免疫荧光检测（CldU IDU）

分析甲基化和其他 S腺苷 L同型半胱氨酸结合蛋白

NanoDrop微量核酸的定量

研究膜蛋白的构象动力学原位网站的荧光标记

天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖

使用微量的生物规格的纳米分光光度计核酸和蛋白的浓度测定

ReAsH / FLASH标签和图像分析Tetracysteine​​传感器蛋白在细胞中

代谢途径的确认和发现通过 13 C - Proteinogenic氨基酸的标签

代谢标记和流式细胞仪测定细胞周期进程的动力学

核酸结构分析及建设3DNA

Read Article