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Chapters
- 00:05Title
- 02:26One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
- 05:07Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
- 08:41Results: Gel Analyses for SMILing and mTAG Reactions
- 11:04Conclusion
DNA en eiwitten sequentie-specifiek gemerkt met affiniteit of fluorescente reporter groepen met DNA of eiwit methyltransferases en synthetische analogen cofactor. Afhankelijk van de cofactor specificiteit van de enzymen, aziridine of dubbel geactiveerde cofactor analogen worden gebruikt voor één of twee stappen labeling.
