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Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
 

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Article DOI: 10.3791/52259-v • 08:39 min • January 3rd, 2015
January 3rd, 2015 •
1,2, 1, 1,2
1Department of Biomedicine, University Hospital Basel, 2Department of Internal Medicine II, University Hospital Tübingen

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요약
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In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

Tags

Stem Cell Properties Ovarian Carcinoma Cells Multi Cell-based Spheres Assays Single Cell-based Spheres Assays Cancer Stem Cells Tumor Initiation Maintenance Relapse Chemotherapies Ovarian CSCs Spheres Assay Non-adherent Culture Conditions Serum-free Medium Growth Factors Sphere Formation Protocols Side-by-side Analysis Multi Cell-based Assays Single Cell Plating Functional Assessment Accurate Results Density Of Plated Cells Titration Experiments Methylcellulose Supplementation Mechanical Artifacts
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