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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
 

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Article DOI: 10.3791/52647-v • 12:49 min • May 25th, 2015
May 25th, 2015 •
1,2, 1,2, 2, 1,2
1School of Life Sciences, Arizona State University, 2Biodesign Institute, Arizona State University

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요약
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Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.

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Keywords: MiRNA Targets 3'LIFE Assay Luminescent Identification 3'UTRs Dual-luciferase Assay Translational Output MiRNA Targeting Non-proprietary Reporter Libraries Genome-wide Screens High-throughput Let-7c MiR-10b
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