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Immunology and Infection

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Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
 

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Article DOI: 10.3791/53265-v 06:20 min December 24th, 2015
December 24th, 2015

챕터

요약

The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.

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Keywords: Mesenchymal Stem Cells Immunomodulation Leukocyte Proliferation CFSE Flow Cytometry Effector Cells Immune-related Diseases
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