Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Detection of Intracellular Gene Expression in…
JoVE Journal
Biology

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biology
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
 

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Article DOI: 10.3791/53268-v • 08:14 min • November 13th, 2015
November 13th, 2015 •
*1, *1, 1, 1, 1, 2, 2,3, 1,4, 1,4
1Department of Cardiovascular Surgery, German Heart Center Munich, Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, 3Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg), Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance
* These authors contributed equally

챕터

요약
Automatic Translation

This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.

Tags

Keywords: Intracellular Gene Expression Live Cells Fluorescence-labeled Nanoparticles Induced Pluripotent Stem Cells Pluripotency Genes Transcription Factors Cell Nucleus Immunohistochemical Staining Gene-specific Nanoparticles Target MRNA Reporter Strand Endocytosis Flow Cytometry
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter