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JoVE Journal Neuroscience
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
 

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Article DOI: 10.3791/53528-v • 16:45 min • March 13th, 2016
March 13th, 2016 •
*1, *1, 2, 1, 3
1Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology, 2Department of Biochemistry, Nencki Institute of Experimental Biology, 3Neurobiology Centre, Nencki Institute of Experimental Biology
* These authors contributed equally

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요약
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This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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Keywords: Two-photon Imaging In Vivo Retrosplenial Cortex Craniotomy Adeno-associated Virus Structural Plasticity GFP MCherry Stereotactic Frame Isoflurane Anesthesia Inflammation Pain Infection
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