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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolatie en Flow cytometrische analyse van Glioom infiltreren perifere mononucleaire bloedcellen
 
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Isolatie en Flow cytometrische analyse van Glioom infiltreren perifere mononucleaire bloedcellen

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Hier voorgesteld is een eenvoudige methode voor het isoleren en flowcytometrische analyse van glioma infiltrerende perifere mononucleaire cellen die tijdsafhankelijk kwantitatieve gegevens over het aantal en activeringsstatus van immuuncellen die het begin hersentumor micro oplevert.

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Immunologie glioom perifere mononucleaire bloedcellen (PBMC's) galectine-1 (gal-1) GL26-Cit-gal1i GL26-Cit-NT Gr-1 natuurlijke killer (NK) -cellen
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