JoVE Journal
Chemistry
Chemistry
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
A high-throughput protocol was developed for combined proteomics and glycomics purification and LC-MS/MS quantification in plasma. Deamidation analysis of N-linked glycosylation motifs was specific to deglycosylated sites. Accurate quantitation of N-glycans was achieved by coupling filter aided N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags strategy.