Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Preparation of 3D Collagen Gels and Microchannels …
JoVE Journal
Bioengineering

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Bioengineering
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
 

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

챕터

요약
Automatic Translation

Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.

Tags

Keywords: 3D Collagen Gels Microchannels 3D Cell Culture Extracellular Matrix Cell Migration Soft Lithography PDMS Neutralization Polymerization Cell Attachment
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter