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Journal / Bioengineering / 3D胶原凝胶和微通道3D相互作用的研究准备…
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Bioengineering

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JoVE Journal Bioengineering
3D胶原凝胶和微通道3D相互作用的研究准备
 
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3D胶原凝胶和微通道3D相互作用的研究准备

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

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요약
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胶原是细胞外基质的核心组件，并提供必要的暗示了几个细胞过程，从移植到分化和增殖。这里提供了一种用于三维胶原凝胶内嵌入电池的协议，和用于产生使用的PDMS微通道的随机或对齐胶原基质的更先进的技术。

Tags

生物工程，111期，胶原蛋白，微流体通道，3D矩阵，ECM，胶原蛋白排列，肿瘤微环境
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