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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
 

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

Article DOI: 10.3791/54168-v • 10:38 min • June 11th, 2016
June 11th, 2016 •
1,2, 3, 3, 3, 1,2, 1,2,7, 1,2, 1,2,4,5,6, 1,2,3,4,5
1Division of Hematology/Oncology, Boston Children’s Hospital, 2Harvard Medical School, 3Division of Hematology, Department of Medicine, Brigham and Women’s Hospital, 4Harvard Stem Cell Institute, 5Broad Institute of Massachusetts Institute of Technology, 6Howard Hughes Medical Institute, 7Division of Hematologic Malignancies, Dana-Farber Cancer Institute

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요약
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This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

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Keywords: Parabiotic Zebrafish Cell Autonomy Hematopoietic System HSC Migration Surgical Fusion Blastula Microinjection Dechorionation Methylcellulose High-calcium Ringer
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